The tutorial of BGMP is as following:
This database encompasses detailed information on the platelet blood group system, red blood cell blood group system, granulocyte blood group system, and Serum Protein (IG) blood group system. It is designed to provide researchers and clinicians with a comprehensive platform for querying and analyzing relevant data, including antigen expression, genetic mutations, and polymorphisms within these blood group systems.
Data scope and curation methods
The database will advance research into blood group systems, support more precise scientific investigations, and assist clinicians in developing more effective treatment plans, thereby reducing complications in transfusions and transplants. Additionally, it will promote global data sharing and collaboration, enhancing research efficiency and improving medical standards.
Here is the usage guide for the database (using the ABO blood group system as an example):
2.1 Use the homepage navigation (A) to open Search, Browse, Submit Data, Downloads, About or Help. Select one of the four blood group system cards (B) to browse Erythrocyte, Platelet (HPA), Granulocyte (HNA) or Serum Protein (IG). Enter a gene, allele, phenotype, variant, rsID or PMID in the search box (C), then click "Search". The "Advanced Search" button beside the search box opens the query builder.
2.2 The Erythrocyte page shows the blood group system introduction (A), the Overview links (B), and the Overview of Database Data Collection (C). Use "10 carbohydrate-based systems (histoblood group systems)" or "40 protein-based systems" in Overview to open a system list. To follow this example, open the carbohydrate-based systems list and select "ABO (ABO)". The search tools and chart gallery remain available on the right.
2.3 The breadcrumb and "ABO Table" heading identify the selected system (A). Use the field filters for Phenotype (B1), Allele (B2), Nucleotide Change (B3), or Amino Acid Change (B4); additional filters include GenBank and PMID. Click the page's "Search" button to apply the field filters. Matching records appear in the results table (C). Use the table controls to change the number of displayed rows, sort columns or move between pages. Scroll the table horizontally to view all columns. The search box above the heading opens a global database search.
Open "Advanced Search" beside the homepage search box or from the category navigation. Select a Search Field (A), such as Gene, Phenotype, Allele, Nucleotide Change, Amino Acid Change, GenBank or PMID, and enter a Search Value (B). Choose AND, OR or NOT (C1), then click "Add to Query" (C2) to build the expression in Query Box (C3). For example, select Gene and enter ABO to create "ABO [Gene]". Click "Search" (D) to display matching records (E). AND combines required conditions, OR allows alternatives, and NOT excludes a condition.
Open "Submit Data" from the navigation. Complete all eight fields in the form (A): blood type system, antigen type, gene mutation, phenotype, GenBank ID, ethnicity information, submitting organization and contact email. The "Submit" button (B) becomes available when all fields are filled. Click it and read the submission result; a successful submission clears the form. If the server reports that data has been saved but email notification failed, do not submit the same record again. The values in this screenshot are illustrative only. "Functional Prediction" (C) opens the external PolyPhen-2 service.
From the Submit Data page, click "Functional Prediction" (C in the screenshot above) to open the external PolyPhen-2 interface. Enter the required protein and substitution information and run the prediction. The existing examples below show the prediction input and output interfaces; the external service may update its interface independently of BGMP.
Prediction results classification: Benign: Indicates that the mutation is likely to have no significant impact on protein function. Possibly Damaging: Indicates that the mutation may have some impact on protein function. Probably Damaging: Indicates that the mutation is likely to have a significant impact on protein function. Score: The model calculates a score ranging from 0 to 1. The higher the score, the greater the potential impact of the mutation on protein function.
Open "Downloads" from the navigation (A). Click the file links in the download table (B) to download the corresponding Erythrocyte, Granulocyte (HNA), Platelet (HPA), or Serum Protein data workbook.
Open "About" from the navigation or "Contact" from the footer, then find "Team, institution and contact" (A). The section lists the maintaining institution, contact person and email address. View contact information. For general feedback, use the Feedback form on the homepage; use Submit Data to contribute a data record.
